7M1C
Crystal structure of the HCMV pentamer-specific antibody 1-32
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2019-08-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 65.677, 65.677, 191.907 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.980 - 2.100 |
Rwork | 0.268 |
R-free | 0.29690 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3u2s |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.200 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 25404 | 13538 |
<I/σ(I)> | 6 | 1.5 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 7.2 | |
CC(1/2) | 0.987 | 0.753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 2.0 M ammonium sulfate, 0.2 M sodium chloride, 5% isopropanol |