7M0O
DGT-28 EPSPS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.978560 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.270, 56.720, 152.189 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.020 - 1.620 |
| R-factor | 0.195 |
| Rwork | 0.194 |
| R-free | 0.22470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gga |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.834 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.095 | 1.648 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Rmerge | 0.083 | 0.849 |
| Rpim | 0.048 | 0.470 |
| Number of reflections | 44171 | 2154 |
| <I/σ(I)> | 17.1 | 2.1 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 7.7 | 8 |
| CC(1/2) | 0.995 | 0.783 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.6 | 277 | 1.2M sodium/potassium phosphate |
