7M0K
HPK1 IN COMPLEX WITH COMPOUND 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.074, 76.627, 89.200 |
| Unit cell angles | 90.02, 97.02, 89.98 |
Refinement procedure
| Resolution | 88.530 - 2.010 |
| R-factor | 0.2483 |
| Rwork | 0.248 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | previously solved structure |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.470 |
| Data scaling software | XSCALE |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.530 | 2.260 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmeas | 0.045 | 0.590 |
| Number of reflections | 74742 | 21707 |
| <I/σ(I)> | 12.37 | 2.15 |
| Completeness [%] | 94.7 | 92.6 |
| Redundancy | 2.2 | 2.2 |
| CC(1/2) | 0.999 | 0.998 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 7% PEG 5K MNE, 0.05 M HEPES pH 6.5, Na3Cit pH 7, 20 mM Tris pH 8.0 , 150 mM NaCl , 5% Glycerol |
