7LZP
LC/A-JPU-B9-JPU-A11-JPU-G11
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-03-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979180 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 132.604, 132.604, 413.030 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 67.060 - 2.860 |
R-factor | 0.2365 |
Rwork | 0.235 |
R-free | 0.27270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3v0a |
RMSD bond length | 0.003 |
RMSD bond angle | 0.584 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 137.680 | 2.960 |
High resolution limit [Å] | 2.860 | 2.860 |
Rpim | 0.088 | 0.893 |
Number of reflections | 50539 | 4409 |
<I/σ(I)> | 12.2 | |
Completeness [%] | 99.6 | |
Redundancy | 9.7 | |
CC(1/2) | 0.998 | 0.405 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M sodium cacodylate pH 6.5, 15 % ethylene glycol, 25 % PEG 4000 |