7LYV
Crystal structure of the HCMV pentamer-specific antibody 1-103
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2016-11-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 2 1 |
Unit cell lengths | 95.389, 105.240, 102.730 |
Unit cell angles | 90.00, 91.37, 90.00 |
Refinement procedure
Resolution | 46.830 - 1.900 |
Rwork | 0.191 |
R-free | 0.21730 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xzc |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.620 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Number of reflections | 143460 | 14384 |
<I/σ(I)> | 5.9 | 1.8 |
Completeness [%] | 89.9 | |
Redundancy | 2 | |
CC(1/2) | 0.996 | 0.736 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 2.1 M sodium formate, 25% PEG 3350, 0.1 M sodium acetate pH 4.5, 0.1 M calcium chloride |