7LXV
Structure of human 20S proteasome with bound MPI-5
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Software | PHENIX,RELION,MotionCorr2,cryoSPARC |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
