7LXS
Structural and Biochemical Insight into Assembly of Molecular Motors Involved in Viral DNA Packaging
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-01 |
| Detector | RIGAKU HyPix-3000 |
| Wavelength(s) | 1.5 |
| Spacegroup name | P 1 |
| Unit cell lengths | 24.747, 50.761, 68.527 |
| Unit cell angles | 90.00, 88.36, 90.11 |
Refinement procedure
| Resolution | 24.740 - 2.210 |
| R-factor | 0.14 |
| Rwork | 0.140 |
| R-free | 0.14200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7lw0 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.740 | 2.290 |
| High resolution limit [Å] | 2.206 | 2.210 |
| Number of reflections | 15881 | 1287 |
| <I/σ(I)> | 93.9 | |
| Completeness [%] | 95.1 | |
| Redundancy | 3.7 | |
| CC(1/2) | 0.999 | 0.999 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 50mM MES pH 6.5, 10mM zinc chloride, 20% PEG 400 |
