7LXE

ENAH EVH1 domain bound to peptide from ABI1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-02-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979180
Spacegroup name	C 2 2 21
Unit cell lengths	56.067, 77.485, 66.167
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.420 - 1.880
R-factor	0.2479
Rwork	0.245
R-free	0.27610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1evh
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	66.170	66.170	1.920
High resolution limit [Å]	1.880	9.000	1.880
Rmerge	0.063	0.039	2.726
Rmeas	0.066	0.040	2.841
Rpim	0.018	0.012	0.788
Total number of observations	157720	1491	9466
Number of reflections	12109	141	757
<I/σ(I)>	15.7	52.5	0.9
Completeness [%]	99.6	99.8	96.6
Redundancy	13	10.6	12.5
CC(1/2)	0.999	0.999	0.702

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M Sodium Acetate pH 4.5, 2.9 M Sodium Chloride