7LVW

Structure of RSV F in Complex with VHH Cl184

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0332
Spacegroup name	P 1 21 1
Unit cell lengths	104.490, 200.560, 134.660
Unit cell angles	90.00, 105.87, 90.00

Refinement procedure

Resolution	50.250 - 2.100
R-factor	0.2025
Rwork	0.201
R-free	0.23040
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5c69
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	89.900	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	301087	15113
<I/σ(I)>	8.4
Completeness [%]	97.4
Redundancy	3.3
CC(1/2)	0.989	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.2 M ammonium citrate pH 4.5, 12.5% (v/v) isopropanol, and 21% (w/v) PEG 3350