7LVS
The CBP TAZ1 Domain in Complex with a CITED2-HIF-1-Alpha Fusion Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-09-07 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97648 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.061, 50.112, 41.255 |
Unit cell angles | 90.00, 99.19, 90.00 |
Refinement procedure
Resolution | 40.730 - 2.020 |
R-factor | 0.2137 |
Rwork | 0.211 |
R-free | 0.24060 |
Structure solution method | AB INITIO PHASING |
RMSD bond length | 0.003 |
RMSD bond angle | 0.645 |
Data scaling software | HKL-2000 |
Phasing software | Arcimboldo |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.730 | 2.146 |
High resolution limit [Å] | 2.000 | 2.019 |
Number of reflections | 8465 | 562 |
<I/σ(I)> | 17.9 | |
Completeness [%] | 100.0 | |
Redundancy | 7.1 | |
CC(1/2) | 0.878 | 0.910 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES pH 6.87, 28% PEG 4000 |