7LUX
AALALL segment from the Nucleoprotein of SARS-CoV-2, residues 217-222, crystal form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 44.460, 9.540, 10.950 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 22.230 - 1.303 |
Rwork | 0.195 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ideal beta strand AAAAA |
RMSD bond length | 0.006 |
RMSD bond angle | 1.179 |
Data reduction software | XDS (Jan 31, 2020) |
Data scaling software | XSCALE (Jan 31, 2020) |
Phasing software | PHASER (2.8.3) |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 22.230 | 22.230 | 1.360 |
High resolution limit [Å] | 1.300 | 4.510 | 1.300 |
Rmerge | 0.105 | 0.065 | 0.808 |
Rmeas | 0.121 | 0.075 | 0.922 |
Number of reflections | 1234 | 35 | 139 |
<I/σ(I)> | 5.94 | 13.6 | 1.8 |
Completeness [%] | 93.0 | 81.4 | 84.8 |
Redundancy | 3.781 | 3.171 | 3.957 |
CC(1/2) | 0.997 | 0.997 | 0.544 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 298 | polyethylene glycol 3000, CHES, pH 9.5 |