7LU6
Crystal structure of the mouse Kirrel3 D1 homodimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-08-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97917 |
Spacegroup name | P 63 |
Unit cell lengths | 63.241, 63.241, 347.809 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.520 - 1.950 |
R-factor | 0.1648 |
Rwork | 0.160 |
R-free | 0.19530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ltw |
RMSD bond length | 0.005 |
RMSD bond angle | 0.684 |
Data reduction software | HKL-2000 |
Phasing software | PHASER (2.6.0) |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.980 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.114 | 0.853 |
Rmeas | 0.130 | 0.970 |
Rpim | 0.062 | 0.459 |
Number of reflections | 57041 | 2865 |
<I/σ(I)> | 14.6 | 1.9 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 4.4 | 4.4 |
CC(1/2) | 0.617 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 0.1 M Sodium cacodylate, pH 6.5, 1.4 M Sodium acetate |