7LU0

X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.88557
Spacegroup name	P 43 21 2
Unit cell lengths	67.752, 67.752, 101.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.880 - 1.010
R-factor	0.1474
Rwork	0.146
R-free	0.16830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ltd
RMSD bond length	0.006
RMSD bond angle	0.894
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.650	1.030
High resolution limit [Å]	1.010	1.010
Rmerge	0.062	1.965
Rpim	0.023	0.922
Number of reflections	123679	5757
<I/σ(I)>	11.8	0.6
Completeness [%]	99.8	95.7
Redundancy	8	5.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate).