7LTW

Crystal structure of the mouse Kirrel2 D1 homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	109.137, 48.444, 44.102
Unit cell angles	90.00, 107.01, 90.00

Refinement procedure

Resolution	32.490 - 1.800
R-factor	0.1562
Rwork	0.155
R-free	0.18090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ofi
RMSD bond length	0.004
RMSD bond angle	0.632
Data reduction software	HKL-2000
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.056	0.173
Rmeas	0.066	0.214
Rpim	0.035	0.124
Number of reflections	19361	715
<I/σ(I)>	21.9	5.3
Completeness [%]	94.2	69.1
Redundancy	3.3	2.6
CC(1/2)		0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.4 M Potassium/Sodium tartrate