7LTD
X-ray radiation damage series on Proteinase K at 100K, crystal structure, dataset 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.88557 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.721, 67.721, 101.423 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.860 - 0.900 |
R-factor | 0.1582 |
Rwork | 0.157 |
R-free | 0.17200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ln7 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.899 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.820 | 0.920 |
High resolution limit [Å] | 0.900 | 0.900 |
Rmerge | 0.076 | 0.716 |
Rpim | 0.029 | 0.519 |
Number of reflections | 173186 | 7909 |
<I/σ(I)> | 10.3 | 1 |
Completeness [%] | 99.6 | 93 |
Redundancy | 6.9 | 2.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Proteinase K was dissolved in 10 mM Calcium Chloride, 50 mM Tris pH 7.5 at a final concentration of 30 mg/ml. 1-2 microliters of this protein solution was mixed with an equivalent volume of precipitant solution (0.5 M Sodium Nitrate). |