7LTA

Galectin-1 in complex with Trehalose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-04-23
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.918
Spacegroup name	P 21 21 21
Unit cell lengths	42.788, 58.090, 112.467
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.400 - 1.530
R-factor	0.2152
Rwork	0.213
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4q26
RMSD bond length	0.010
RMSD bond angle	1.132
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.18.2_3874)
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.400	1.620
High resolution limit [Å]	1.530	1.530
Number of reflections	43161	6807
<I/σ(I)>	10.37
Completeness [%]	99.9
Redundancy	6.52
CC(1/2)	0.990	0.530

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	PEG 4000