7LT8
Crystal structure of Ras suppressor-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-08-17 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97919 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.851, 45.328, 83.052 |
Unit cell angles | 90.00, 99.02, 90.00 |
Refinement procedure
Resolution | 27.341 - 1.760 |
R-factor | 0.175516614945 |
Rwork | 0.173 |
R-free | 0.21666 |
Structure solution method | SAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.814 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELX |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.790 |
High resolution limit [Å] | 1.760 | 1.760 |
Rmerge | 0.084 | 0.804 |
Number of reflections | 22975 | 1136 |
<I/σ(I)> | 36.52 | |
Completeness [%] | 97.1 | |
Redundancy | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 296 | PEG4000, lithium chloride |