7LT5

CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Cofactor SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	81.418, 160.856, 230.161
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.100 - 2.540
R-factor	0.1779
Rwork	0.177
R-free	0.21150
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7lnj
RMSD bond length	0.002
RMSD bond angle	0.491
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.100	2.640
High resolution limit [Å]	2.540	2.540
Rmerge	0.179	1.498
Rpim	0.058	0.478
Number of reflections	88557	8168
<I/σ(I)>	10.7	1.4
Completeness [%]	88.8	83.1
Redundancy	9.1
CC(1/2)		0.564

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	292	0.1 M Tris-HCl pH 7.3, 23% PEG 3350, 0.27 M Potassium citrate