7LT1

Structure of the cGAS-like receptor human MB21D2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2018-10-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97918
Spacegroup name	I 2 2 2
Unit cell lengths	102.170, 127.822, 198.235
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.130 - 2.400
R-factor	0.2479
Rwork	0.247
R-free	0.26730
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.390
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.560	2.480
High resolution limit [Å]	2.400	2.400
Rpim	0.038	0.823
Number of reflections	50814	4680
<I/σ(I)>	12.6	1.3
Completeness [%]	99.6
Redundancy	7
CC(1/2)	0.999	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	1.2 M ammonium sulfate, 5 mM magnesium chloride, 100 mM MES, pH 6.2