7LSI
Structure of KD035, a VEGFR2 monoclonal antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU |
Temperature [K] | 62 |
Detector technology | PIXEL |
Collection date | 2016-02-02 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 53.164, 90.236, 81.529 |
Unit cell angles | 90.00, 93.81, 90.00 |
Refinement procedure
Resolution | 39.456 - 2.400 |
R-factor | 0.2 |
Rwork | 0.197 |
R-free | 0.27420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3n9g |
RMSD bond length | 0.010 |
RMSD bond angle | 1.360 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.460 | 2.060 |
High resolution limit [Å] | 1.990 | 1.990 |
Number of reflections | 50743 | 4626 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 96.5 | |
Redundancy | 3.3 | |
CC(1/2) | 0.900 | 0.310 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 0.2M Na Phosphate monobasic monohydrate,20% PEG3350 |