7LSA
Ruminococcus bromii Amy12 with maltoheptaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2015-02-11 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.977, 97.960, 167.089 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.460 - 1.760 |
| R-factor | 0.1604 |
| Rwork | 0.159 |
| R-free | 0.20640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wan |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.992 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.460 | 1.820 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 74259 | 5901 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 95.9 | |
| Redundancy | 4.7 | |
| CC(1/2) | 0.990 | 0.260 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | well solution: 16% PEG 3350, 4% glycerol, 0.3 ammonium acetate, and 0.1 M Bis-Tris pH 6.5; protein: 8.75 - 8.9 mg/ml Amy12 with 10mM maltoheptaose |
