7LRM

Structure of HIV-1 Reverse Transcriptase in complex with DNA, dCTP, and CA(2+) ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-31
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977
Spacegroup name	P 1 21 1
Unit cell lengths	89.663, 131.247, 137.491
Unit cell angles	90.00, 97.72, 90.00

Refinement procedure

Resolution	34.130 - 3.140
R-factor	0.1962
Rwork	0.194
R-free	0.24370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5d3g
RMSD bond length	0.002
RMSD bond angle	0.582
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.200
High resolution limit [Å]	3.140	3.140
Rmerge	0.088	0.499
Rmeas	0.097	0.590
Rpim	0.039	0.305
Number of reflections	53976	2194
<I/σ(I)>	16.2	1.5
Completeness [%]	98.0	79.8
Redundancy	5.5	2.9
CC(1/2)	0.914	0.574

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	277	10-12% PEG 8000, 50 MM BISTRIS-PROPANE PH 7.2, 50 MM AMMONIUM SULFATE, 5% GLYCEROL, 5% SUCROSE