7LRH

C-terminal domain of RibD from Brucella abortus (5-amino-6-ribosylamino-2,4(1H,3H)-pyrimidinedione 5'-phosphate reductase)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-26
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 1
Unit cell lengths	54.910, 56.700, 79.600
Unit cell angles	95.99, 91.25, 113.16

Refinement procedure

Resolution	50.360 - 1.920
R-factor	0.2361
Rwork	0.234
R-free	0.27410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b3z
RMSD bond length	0.014
RMSD bond angle	1.644
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	BALBES
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.360	1.970
High resolution limit [Å]	1.920	1.920
Rmerge	0.085	2.624
Rmeas	0.089	2.738
Rpim	0.026	0.779
Number of reflections	65607	4379
<I/σ(I)>	12.1	1
Completeness [%]	98.1	96.9
Redundancy	11.8	12.2
CC(1/2)	0.999	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2	294	12% (w/v) PEG 8000 + 0.1 M MES pH 6,2 + 0.2 M ammonium sulfate