7LQO

Crystal structure of a genetically encoded red fluorescent peroxynitrite biosensor, pnRFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-29
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.115834
Spacegroup name	C 1 2 1
Unit cell lengths	84.203, 34.567, 88.815
Unit cell angles	90.00, 110.90, 90.00

Refinement procedure

Resolution	41.810 - 2.100
R-factor	0.1908
Rwork	0.188
R-free	0.25390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5f9g
RMSD bond length	0.008
RMSD bond angle	1.571
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.810	2.160
High resolution limit [Å]	2.100	2.100
Number of reflections	13553	831
<I/σ(I)>	13.3
Completeness [%]	95.1
Redundancy	3.74
CC(1/2)	0.998	0.805

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288	Sodium Phosphate, citric acid, PEG 400.