7LQM
Glucosamie-6-phosphate Deaminase from Pasturella multocida
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-07-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9786 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 84.796, 79.569, 85.295 |
Unit cell angles | 90.00, 109.13, 90.00 |
Refinement procedure
Resolution | 37.100 - 2.300 |
R-factor | 0.2019 |
Rwork | 0.200 |
R-free | 0.23170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dea |
Data reduction software | XDS (Jan 26, 2018) |
Data scaling software | Aimless (0.6.3) |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 41.930 | 41.930 | 2.380 |
High resolution limit [Å] | 2.300 | 8.910 | 2.300 |
Rmerge | 0.036 | 0.021 | 0.203 |
Rmeas | 0.043 | 0.026 | 0.247 |
Rpim | 0.023 | 0.015 | 0.139 |
Total number of observations | 156289 | 2529 | 13418 |
Number of reflections | 47456 | 838 | 4648 |
<I/σ(I)> | 17.7 | 43.9 | 4.4 |
Completeness [%] | 99.3 | 96.6 | 99.5 |
Redundancy | 3.3 | 3 | 2.9 |
CC(1/2) | 0.998 | 0.994 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 100 mM Sodium Cacodylate/HCl pH 6.5, 200 mM Magnesium Chloride, 20 % PEG 1000. |