7LQ4

Rr (RsiG)2-(c-di-GMP)2-WhiG complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-01-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.01
Spacegroup name	P 21 21 21
Unit cell lengths	46.466, 81.621, 114.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.480 - 2.900
R-factor	0.2673
Rwork	0.267
R-free	0.26830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pfj
RMSD bond length	0.002
RMSD bond angle	0.477
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.480	3.040
High resolution limit [Å]	2.900	2.930
Rpim	0.035	0.230
Number of reflections	9237	601
<I/σ(I)>	17.1
Completeness [%]	91.9
Redundancy	2.9
CC(1/2)	0.999	0.804

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris pH 8.0, 0.2 M MgCl2, 23% PEG 3350