7LQ3
Evolution of a sigma-(c-di-GMP)-antisigma switch
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 78.545, 78.545, 81.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.273 - 2.550 |
R-factor | 0.2057 |
Rwork | 0.199 |
R-free | 0.26840 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lq2 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.273 | 2.590 |
High resolution limit [Å] | 2.550 | 2.550 |
Rpim | 0.028 | 0.453 |
Number of reflections | 8738 | 847 |
<I/σ(I)> | 20.2 | 2.9 |
Completeness [%] | 99.6 | |
Redundancy | 10.5 | |
CC(1/2) | 0.999 | 0.804 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M sodium acetate pH 4.5, 2.5 M NaCl, 0.2 M lithium sulfate, 0.1 M MgCl2 |