7LQ2
Apo Rr RsiG- crystal form 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-06-27 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 59.479, 95.293, 117.599 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.457 - 1.850 |
R-factor | 0.1745 |
Rwork | 0.174 |
R-free | 0.20220 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.460 | 1.920 |
High resolution limit [Å] | 1.850 | 1.850 |
Rpim | 0.032 | 0.374 |
Number of reflections | 57371 | 5313 |
<I/σ(I)> | 17.8 | 2.4 |
Completeness [%] | 99.6 | |
Redundancy | 6.6 | |
CC(1/2) | 0.998 | 0.898 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 12% isopropanol, 0.1 M HEPES pH 7.5, 0.1 M MgCl2 |