7LPZ
Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-01-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.727, 74.785, 96.879 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.390 - 1.550 |
R-factor | 0.1596 |
Rwork | 0.158 |
R-free | 0.19680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xri |
RMSD bond length | 0.005 |
RMSD bond angle | 0.772 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.390 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Number of reflections | 407820 | 63859 |
<I/σ(I)> | 22.42 | |
Completeness [%] | 99.4 | |
Redundancy | 6.56 | |
CC(1/2) | 1.000 | 0.631 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 290 | 30% PEG 3350, 25% saturated KCl, 200 mM Imidazole / Malate buffer 8.0, 2.5 mM samarium (III) acetate, 18mM AMP, 3.3 mM N-{2-[4-(thiophen-2-yl)-1H-imidazol-2-yl]ethyl}-2,3-dihydro-1,4-benzodioxine-2-carboxamide (from Life Chemicals) |