7LPO
Crystal structure of Cryptococcus neoformans sterylglucosidase 1 with tris
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-1 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-1 |
Temperature [K] | 150 |
Detector technology | PIXEL |
Collection date | 2019-06-25 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 98.597, 126.606, 126.655 |
Unit cell angles | 90.00, 95.46, 90.00 |
Refinement procedure
Resolution | 47.280 - 2.130 |
R-factor | 0.159 |
Rwork | 0.157 |
R-free | 0.19760 |
Structure solution method | SAD |
Data reduction software | Aimless |
Data scaling software | xia2 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1-3660) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.280 | 47.280 | 2.170 |
High resolution limit [Å] | 2.130 | 11.670 | 2.130 |
Rmerge | 0.111 | 0.033 | 0.593 |
Rmeas | 0.133 | 0.039 | 0.757 |
Rpim | 0.091 | 0.022 | 0.463 |
Number of reflections | 171606 | 1095 | 7694 |
<I/σ(I)> | 6.5 | 15 | 1.4 |
Completeness [%] | 99.4 | 98.4 | 90.5 |
Redundancy | 3.2 | 3.3 | 2.3 |
CC(1/2) | 0.969 | 0.990 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12 % PEG8000, 0.2M MgCl2, 0.1 M Tris 7.5 |