7LPJ
APE1 Mn-bound phosphorothioate substrate complex with abasic ribonucleotide DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-09-21 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.171, 60.901, 73.320 |
| Unit cell angles | 82.91, 77.58, 86.25 |
Refinement procedure
| Resolution | 48.820 - 2.560 |
| R-factor | 0.1888 |
| Rwork | 0.183 |
| R-free | 0.24810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5dff |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.306 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.610 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Rmeas | 0.146 | |
| Number of reflections | 31441 | 1184 |
| <I/σ(I)> | 12.3 | |
| Completeness [%] | 99.8 | 97.7 |
| Redundancy | 5.6 | 4.4 |
| CC(1/2) | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4 | 291 | 50 mM tri-Sodium Citrate (pH 4), 100 mM NaCl, 10% Peg 6,000 |
