7LP2

Structure of Nedd4L WW3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-01-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9753
Spacegroup name	P 4 21 2
Unit cell lengths	91.598, 91.598, 37.982
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.980 - 1.880
R-factor	0.1896
Rwork	0.185
R-free	0.22790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2mpt
RMSD bond length	0.008
RMSD bond angle	0.958
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.990	1.781
High resolution limit [Å]	1.720	1.720
Rmerge	0.023	0.590
Rmeas	0.331	0.033
Number of reflections	13684	1731
<I/σ(I)>	20.61	1.22
Completeness [%]	99.9	95.77
Redundancy	2	2
CC(1/2)	1.000	0.449

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	2.0M Ammonium Sulfate, 0.1M BIS-TRIS