7LP1

Structure of Nedd4L WW3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL7-1
Synchrotron site	SSRL
Beamline	BL7-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1
Spacegroup name	P 31 2 1
Unit cell lengths	40.344, 40.344, 54.860
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.470 - 1.350
R-factor	0.1868
Rwork	0.184
R-free	0.21350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2mpt
RMSD bond length	0.010
RMSD bond angle	1.116
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.398
High resolution limit [Å]	1.000	1.350
Rmerge		0.169
Rmeas		0.238
Number of reflections	11774	1140
<I/σ(I)>	33.25	4.26
Completeness [%]	99.8	98.16
Redundancy	2	2
CC(1/2)	1.000	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	293.15	3.6M ammonium nitrate, 5% glycerol, and 0.1M sodium acetate at pH 4.6