7LOO
S-adenosyl methionine transferase cocrystallized with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.922, 117.780, 113.210 |
| Unit cell angles | 90.00, 107.52, 90.00 |
Refinement procedure
| Resolution | 35.990 - 1.950 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p7l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.881 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.790 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.151 | |
| Number of reflections | 123360 | 6027 |
| <I/σ(I)> | 8.2 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7 | |
| CC(1/2) | 0.998 | 0.445 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.1 M Bis-Tris 6.5, 15% ethylene glycol, PEG 3350 20% |
