7LNV

Apo Structure of Isopentenyl Phosphate Kinase from Candidatus methanomethylophilus alvus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-25
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	194.392, 45.630, 113.874
Unit cell angles	90.00, 121.20, 90.00

Refinement procedure

Resolution	37.290 - 2.200
R-factor	0.2303
Rwork	0.228
R-free	0.28140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lkk
RMSD bond length	0.003
RMSD bond angle	0.520
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.240
High resolution limit [Å]	2.200	2.200
Number of reflections	44070	2159
<I/σ(I)>	9.91
Completeness [%]	99.8
Redundancy	3.9
CC(1/2)	0.997	0.552

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	0.2 M Sodium Malonate pH 7.0, 20% PEG 3350.