7LNN

E. coli S-adenosyl methionine transferase co-crystallized with guanosine-5'-imidotriphosphate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 61 2 2
Unit cell lengths	122.336, 122.336, 287.267
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.630 - 2.500
R-factor	0.2233
Rwork	0.222
R-free	0.24260
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1p7l
RMSD bond length	0.002
RMSD bond angle	0.573
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.630	2.590
High resolution limit [Å]	2.500	2.500
Number of reflections	44910	4600
<I/σ(I)>	16.2
Completeness [%]	100.0
Redundancy	39.1
CC(1/2)	0.999	0.675

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	8% OEG 8000, 0.1 M Bis-Tris pH 6.5, 20% ethylene glycol