7LNJ
CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-11-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 82.253, 161.361, 230.633 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.350 - 2.680 |
R-factor | 0.2123 |
Rwork | 0.210 |
R-free | 0.25070 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 7lni |
RMSD bond length | 0.002 |
RMSD bond angle | 0.431 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.350 | 2.790 |
High resolution limit [Å] | 2.680 | 2.680 |
Rmerge | 0.224 | 2.040 |
Rpim | 0.068 | 0.690 |
Number of reflections | 75973 | 6312 |
<I/σ(I)> | 16.3 | 0.9 |
Completeness [%] | 87.8 | 73.8 |
Redundancy | 10.3 | 7 |
CC(1/2) | 0.384 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 292 | 0.1 M Bis-Tris pH 6.7, 23% polyethylene glycol (PEG) 3350, 0.3 M potassium citrate |