7LNG
TNA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2021-02-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033175 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 45.165, 45.165, 82.831 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 82.830 - 2.200 |
Rwork | 0.225 |
R-free | 0.27470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uee |
RMSD bond length | 0.017 |
RMSD bond angle | 2.663 |
Data reduction software | xia2 |
Data scaling software | DIALS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 82.830 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.103 | 0.295 |
Rmeas | 0.111 | 0.312 |
Rpim | 0.040 | 0.101 |
Number of reflections | 5325 | 266 |
<I/σ(I)> | 20 | 1.3 |
Completeness [%] | 99.7 | 100 |
Redundancy | 8.9 | |
CC(1/2) | 0.996 | 0.955 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES sodium pH 7.5, 1.5 M Lithium sulfate monohydrate |