7LNE
ANA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 99 |
Detector technology | PIXEL |
Collection date | 2021-02-05 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033175 |
Spacegroup name | P 3 2 1 |
Unit cell lengths | 43.408, 43.408, 86.554 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 37.621 - 1.530 |
Rwork | 0.191 |
R-free | 0.22540 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5uee |
RMSD bond length | 0.025 |
RMSD bond angle | 3.091 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.560 |
High resolution limit [Å] | 1.530 | 1.530 |
Rmerge | 0.066 | 0.198 |
Rmeas | 0.073 | 0.226 |
Rpim | 0.031 | 0.106 |
Number of reflections | 14626 | 691 |
<I/σ(I)> | 19.9 | 6.1 |
Completeness [%] | 98.7 | 96.9 |
Redundancy | 5.8 | 3.4 |
CC(1/2) | 0.999 | 0.973 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.7 M Lithium sulfate, 10 % w/v Glycerol |