7LNE

ANA modification at 3' end of RNA primer complex with guanosine dinucleotide ligand G(5')ppp(5')G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	99
Detector technology	PIXEL
Collection date	2021-02-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033175
Spacegroup name	P 3 2 1
Unit cell lengths	43.408, 43.408, 86.554
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.621 - 1.530
Rwork	0.191
R-free	0.22540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5uee
RMSD bond length	0.025
RMSD bond angle	3.091
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.560
High resolution limit [Å]	1.530	1.530
Rmerge	0.066	0.198
Rmeas	0.073	0.226
Rpim	0.031	0.106
Number of reflections	14626	691
<I/σ(I)>	19.9	6.1
Completeness [%]	98.7	96.9
Redundancy	5.8	3.4
CC(1/2)	0.999	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.7 M Lithium sulfate, 10 % w/v Glycerol