7LMW

Receptor for Advanced Glycation End Products VC1 domain in complex with 3-(3-((4-(4-carboxyphenoxy)benzyl)oxy)phenyl)-1H-indole-2-carboxylic acid

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0781
Spacegroup name	P 62
Unit cell lengths	101.885, 101.885, 102.306
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.410 - 2.500
R-factor	0.2031
Rwork	0.200
R-free	0.25610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lp4
RMSD bond length	0.012
RMSD bond angle	1.411
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.820
High resolution limit [Å]	2.500	2.500
Rmeas	0.086	0.461
Rpim	0.037	0.199
Number of reflections	20999	6412
<I/σ(I)>	13	2.6
Completeness [%]	99.8	100
Redundancy	2.7	2.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	2.5 M NaOAc (pH 7.5)