7LMK
Crystal structure of bovine DNMT1 BAH1 domain in complex with H4K20me3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.98 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 71.060, 81.372, 129.653 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 71.060 - 2.647 |
R-factor | 0.2106 |
Rwork | 0.206 |
R-free | 0.25830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4wxx |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.370 | 2.780 |
High resolution limit [Å] | 2.647 | 2.650 |
Number of reflections | 22223 | 2882 |
<I/σ(I)> | 15.7 | |
Completeness [%] | 98.8 | |
Redundancy | 4.4 | |
CC(1/2) | 0.998 | 0.404 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 13% PEG1500, 20 mM DTT and 200 mM L-proline, 0.1 M HEPES (pH 7.5) |