7LM3
Crystal Structure of Thr316Ala mutant of JAMM domain of S. pombe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-08-01 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.531, 121.338, 57.145 |
| Unit cell angles | 90.00, 129.58, 90.00 |
Refinement procedure
| Resolution | 44.040 - 2.700 |
| R-factor | 0.2033 |
| Rwork | 0.201 |
| R-free | 0.25290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4jxe |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.139 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (19.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.750 |
| High resolution limit [Å] | 2.700 | 7.320 | 2.700 |
| Rmerge | 0.074 | 0.028 | 0.721 |
| Rmeas | 0.086 | 0.033 | 0.838 |
| Rpim | 0.043 | 0.017 | 0.425 |
| Total number of observations | 49946 | ||
| Number of reflections | 12793 | 668 | 616 |
| <I/σ(I)> | 9 | ||
| Completeness [%] | 98.8 | 99.1 | 97.5 |
| Redundancy | 3.9 | 3.8 | 3.9 |
| CC(1/2) | 0.999 | 0.786 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.2 M ammonium phosphate dibasic (pH 8.0), 20% w/v polyethylene glycol 3,350 with 40% v/v Polypropylene glycol P400 as an additive |
