7LL0
Crystal structure of a four-tetrad, parallel, and K+ stabilized Tetrahymena thermophila telomeric G-quadruplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 196 |
Detector technology | CCD |
Collection date | 2020-11-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97918 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 30.090, 36.474, 53.253 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.090 - 2.000 |
R-factor | 0.2168 |
Rwork | 0.214 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7jku |
RMSD bond length | 0.010 |
RMSD bond angle | 1.045 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.18_3845) |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 53.250 | 2.050 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.069 | |
Rmeas | 0.074 | |
Rpim | 0.025 | 0.753 |
Number of reflections | 4313 | 299 |
<I/σ(I)> | 12 | 0.5 |
Completeness [%] | 99.6 | 95.8 |
Redundancy | 8.9 | 8.4 |
CC(1/2) | 0.999 | 0.418 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | sodium cacodylate, KCl, PEG, NaCl, ethylene glycol |