7LKQ

The PilZ(delta107-117)-FimX(GGDEF-EAL) complex from Xanthomonas citri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-03-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54187
Spacegroup name	P 42 21 2
Unit cell lengths	96.100, 96.100, 146.334
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.790 - 3.400
R-factor	0.2454
Rwork	0.244
R-free	0.28130
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fou
RMSD bond length	0.010
RMSD bond angle	1.184
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.740
High resolution limit [Å]	3.400	3.400
Rmerge	0.070	0.555
Number of reflections	9964	2287
<I/σ(I)>	11	3.3
Completeness [%]	100.0	100
Redundancy	13.6	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	10 mg/ml protein, 0.1 M MES pH 6.5, 1.6 M MgSO4