7LKO
The PilB(N-terminal)-PilZ complex of the Type IV pilus from Xanthomonas citri (2.9 A)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2015-01-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54187 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.353, 123.547, 63.539 |
Unit cell angles | 90.00, 99.28, 90.00 |
Refinement procedure
Resolution | 44.010 - 2.880 |
R-factor | 0.1616 |
Rwork | 0.158 |
R-free | 0.22720 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PilB-PilZ SeMet model |
RMSD bond length | 0.013 |
RMSD bond angle | 1.454 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.010 | 2.980 |
High resolution limit [Å] | 2.880 | 2.880 |
Rmerge | 0.060 | 0.190 |
Number of reflections | 13503 | 2614 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 99.6 | 97.6 |
Redundancy | 2.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 4 mg/ml protein, 0.1 M Tris pH 8.5, 2.0 M ammonium sulfate |