7LKN

The PilB(N-terminal_P70S mutant)-PilZ complex (SeMet)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-08-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97889
Spacegroup name	P 1 21 1
Unit cell lengths	39.056, 122.340, 62.394
Unit cell angles	90.00, 98.62, 90.00

Refinement procedure

Resolution	30.720 - 1.710
R-factor	0.2088
Rwork	0.206
R-free	0.25420
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.163
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AutoSol
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.720	1.810
High resolution limit [Å]	1.710	1.710
Rmerge	0.080	0.790
Number of reflections	62185	8896
<I/σ(I)>	8.78
Completeness [%]	99.4	97.7
Redundancy	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	291	0.1 M Tris pH 8.5; 2.0 M ammonium sulfate