7LKN
The PilB(N-terminal_P70S mutant)-PilZ complex (SeMet)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-08-12 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.97889 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.056, 122.340, 62.394 |
Unit cell angles | 90.00, 98.62, 90.00 |
Refinement procedure
Resolution | 30.720 - 1.710 |
R-factor | 0.2088 |
Rwork | 0.206 |
R-free | 0.25420 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 1.163 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.720 | 1.810 |
High resolution limit [Å] | 1.710 | 1.710 |
Rmerge | 0.080 | 0.790 |
Number of reflections | 62185 | 8896 |
<I/σ(I)> | 8.78 | |
Completeness [%] | 99.4 | 97.7 |
Redundancy | 1.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Tris pH 8.5; 2.0 M ammonium sulfate |