7LKM
The PilB(N-terminal_P70S mutant)-PilZ complex of the Type IV pilus from Xanthomonas citri
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-12 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.45866 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.063, 122.408, 62.730 |
| Unit cell angles | 90.00, 98.98, 90.00 |
Refinement procedure
| Resolution | 28.000 - 2.000 |
| R-factor | 0.1815 |
| Rwork | 0.179 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PilB-PilZ_SeMet_Model |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | REFMAC |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.000 | 3.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.150 | 1.250 |
| Number of reflections | 44979 | 4457 |
| <I/σ(I)> | 10.1 | |
| Completeness [%] | 99.6 | |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.1 M Tris pH 8.5; 2.0 M ammonium sulfate |
