7LKG
Crystal structure of PfCSP peptide 21 with vaccine-elicited human anti-malaria antibody m43.151
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 54.421, 54.169, 75.701 |
Unit cell angles | 77.62, 77.09, 67.59 |
Refinement procedure
Resolution | 38.380 - 2.020 |
R-factor | 0.2148 |
Rwork | 0.213 |
R-free | 0.25350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6b5m |
RMSD bond length | 0.003 |
RMSD bond angle | 0.705 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (dev_3936) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.090 |
High resolution limit [Å] | 2.020 | 5.560 | 2.050 |
Rmerge | 0.134 | 0.048 | 0.480 |
Rmeas | 0.170 | 0.062 | 0.618 |
Rpim | 0.103 | 0.038 | 0.385 |
Number of reflections | 43488 | 1899 | 2295 |
<I/σ(I)> | 6.6 | ||
Completeness [%] | 87.8 | 76.5 | 90.6 |
Redundancy | 2.5 | 2.3 | 2.4 |
CC(1/2) | 0.992 | 0.647 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Citric acid pH 3.5 and 25% w/v polyethylene glycol 3350 |