7LJM
Structure of the Salmonella enterica CD-NTase CdnD in complex with GTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2019-08-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 74.473, 69.781, 88.697 |
Unit cell angles | 90.00, 110.35, 90.00 |
Refinement procedure
Resolution | 38.490 - 2.600 |
R-factor | 0.2318 |
Rwork | 0.229 |
R-free | 0.26530 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | EcCdnD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.657 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.490 | 2.710 |
High resolution limit [Å] | 2.600 | 2.600 |
Rpim | 0.135 | 0.965 |
Number of reflections | 26345 | 3093 |
<I/σ(I)> | 3.5 | 1.1 |
Completeness [%] | 99.3 | |
Redundancy | 3.5 | |
CC(1/2) | 0.985 | 0.381 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M MES pH 6.5, 25% PEG-3000 |