7LJA
Human TRAAK K+ channel FHEIG mutant A198E in a Tl+ bound conductive conformation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2019-10-29 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9778 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 80.230, 136.530, 95.280 |
Unit cell angles | 90.00, 94.54, 90.00 |
Refinement procedure
Resolution | 47.540 - 2.770 |
R-factor | 0.2034 |
Rwork | 0.201 |
R-free | 0.24340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7lj5 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.590 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.540 | 3.021 |
High resolution limit [Å] | 2.770 | 2.770 |
Rmerge | 3.376 | |
Rmeas | 3.438 | |
Rpim | 0.648 | |
Number of reflections | 35508 | 2217 |
<I/σ(I)> | 9.8 | 1.338 |
Completeness [%] | 93.4 | 19.56 |
Redundancy | 27.7 | 14.03 |
CC(1/2) | 0.995 | 0.581 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 277 | 50mM Tris pH 8.8, 64-200 mM CaCl2, 27-33% (vol/vol) PEG400 |